Surface Modification of Thin Polyion Complex Film with a High Specific Binding Affinity and Prevention of Non-specific Adsorption in Surface Plasmon Resonance Immunoassay
نویسندگان
چکیده
منابع مشابه
Reducing Non-Specific Binding in Surface Plasmon Resonance Experiments
Non-specific binding is an important experimental parameter to control when using SPR systems. Non-specific binding is the binding of analyte to non-target molecules on the sensor surface, as illustrated in Figure 1. The effect of non-specific interactions is a false positive contribution to the signal in a sensorgram. It is important for users to recognize non-specific binding and to implement...
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Thanks to resonant excitation of surface plasmons, SPR sensors including ferromagnetic thin films allow the enhancement of the magneto-optical response. MO-SPR structures with Au/Co/Au sandwiches are theoretically analyzed in transverse configuration. The influence of sandwich film thicknesses and layer order is discussed. The optimized geometry and corresponding feedback are specified.
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We fabricated a surface plasmon resonance (SPR) sensor using a hydrophilic polymer for the highly sensitive detection of 2,4,6-trinitrotoluene (TNT). The hydrophilic polymer was made from mono-2-(methacryloyloxy)ethylsuccinate (MES) and 2-hydroxyethylmethacrylate (HEMA) by surface-initiated atom transfer radical polymerization. The detection of TNT was carried out by displacement assay with the...
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The aim of this study is experimental assay of sensitivity and stability of a bimetallic silver/gold SPR sensor chip. This chip utilizes the sensitivity of the silver and the stability of the gold. Moreover, the Silver layer (instead of usual Cr or Ti layer) was used as an adhesive intermediate layer between the Gold layer and the glass substrate. The optimization of the Gold/Silver thickness u...
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امروزه سالیتون ها بعنوان امواج جایگزیده ای که تحت شرایط خاص بدون تغییر شکل در محیط منتشر می-شوند، زمینه مطالعات گسترده ای در حوزه اپتیک غیرخطی هستند. در این راستا توجه به پدیده پراش گسسته، که بعنوان عامل پهن شدگی باریکه نوری در آرایه ای از موجبرهای جفت شده، ظاهر می گردد، ضروری است، زیرا سالیتون های گسسته از خنثی شدن پراش گسسته در این سیستم ها بوسیله عوامل غیرخطی بوجود می آیند. گسستگی سیستم عامل...
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ژورنال
عنوان ژورنال: Electrochemistry
سال: 2006
ISSN: 2186-2451,1344-3542
DOI: 10.5796/electrochemistry.74.121